Active Recombinant Rat Angpt2 protein, His-tagged, Low Endotoxin

• Cat.No.: ANGPT2-325R
• Product Overview: Recombinant Rat Angpt2 protein(O35462)(275-496 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 275-496 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat ANGPT2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.28 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Angpt2 angiopoietin 2 [ Rattus norvegicus ]
• Official Symbol: Angpt2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Agpt2; Ang-2
• Gene ID: 89805
• mRNA Refseq: NM_134454
• Protein Refseq: NP_604449