Recombinant Rat Atg10 Protein, His-tagged

• Cat.No.: Atg10-258R
• Product Overview: Recombinant Rat Atg10 Protein (Met1-Glu214) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable Atg12 conjugating enzyme activity. Predicted to be involved in ER overload response; cellular protein modification process; and macroautophagy. Predicted to act upstream of or within positive regulation of protein modification process. Orthologous to human ATG10 (autophagy related 10).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.1 kDa
  Accurate Molecular Mass: 30 kDa
• Protein length: Met1-Glu214
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Atg10 autophagy related 10 homolog (S. cerevisiae) [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Atg10
• Synonyms: ATG10; autophagy related 10 homolog (S. cerevisiae); autophagy-related protein 10; autophagy-related 10
• Gene ID: 688555
• mRNA Refseq: NM_001109505
• Protein Refseq: NP_001102975
• UniProt ID: D4A9K6