Recombinant Rat CA5a Protein, His (Fc)-Avi-tagged

• Cat.No.: CA5a-719R
• Product Overview: Recombinant Rat CA5a with His (Fc)-Avi tag was expressed and purified

Specification

• Source: HEK293
• Species: Rat
• Tag: His&Fc&Avi
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥85% by SDS-PAGE
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Car5a carbonic anhydrase 5a, mitochondrial [ Rattus norvegicus ]
• Official Symbol: CAR5A
• Gene ID: 54233
• mRNA Refseq: NM_019293.2
• Protein Refseq: NP_062166.1
• UniProt ID: P43165

Reviews

08/26/2022

Efficient protein quantification, saves time and resources.

01/15/2022

Consistency in results, a symbol of their service quality.

05/25/2021

Top-tier protein sequencing, propels our research forward.

Q&As

What post-translational modifications are observed in CA5A?

Post-translational modifications of CA5A can be identified using mass spectrometry, with potential phosphorylation or acetylation sites.

What diseases or conditions are associated with CA5A dysregulation?

Dysregulation of CA5A might influence metabolic processes in the liver, potentially impacting conditions like fatty liver disease or other metabolic disorders.

Are there genetic mutations in the CA5A gene that impact its function?

Specific mutations might affect CA5A's stability or activity, necessitating functional assays for validation.

Are there specific compounds known to inhibit or activate CA5A activity?

While general carbonic anhydrase inhibitors might affect CA5A, specific modulators would need further research.

What is the primary function of CA5A in cellular processes?

CA5A facilitates the reversible conversion of CO2 to bicarbonate and protons, playing a role in pH regulation and CO2 transport.

How does CA5A's enzymatic activity compare with other carbonic anhydrase isoforms?

CA5A's activity is tailored to its mitochondrial environment, which might differ in kinetics from cytosolic isoforms.

How does the expression profile of CA5A differ among human tissues?

CA5A is primarily expressed in the mitochondria of liver cells, with lesser amounts in other tissues.