Recombinant Rat Chemokine (C-C Motif) Ligand 4

• Cat.No.: Ccl4-106R
• Product Overview: Recombinant rat MIP-1 beta produced inE.coliis a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Specification

• Cat. No.: Ccl4-106R
• Description: Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.
• Source: E.coli.
• Purity: > 98% by SDS-PAGE and HPLC analysis.
• Endotoxin level: < 0.1 ng/μg of MIP-1 beta.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: Determined by its ability to chemoattract human monocytes using a concentration range of 0.01-1.0 μg/ml.
• Reconstitution: The rat MIP-1 beta should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
• Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted rat MIP-1 beta should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Ccl4 chemokine (C-C motif) ligand 4 [ Rattus norvegicus ]
• Synonyms: Ccl4; chemokine (C-C motif) ligand 4; Scya4; Mip1-b; small inducible cytokine A4; macrophage inflammatory protein-1 beta
• Gene ID: 116637
• mRNA Refseq: NM_053858
• Protein Refseq: NP_446310
• UniProt ID: P50230
• Chromosome Location: q26
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction
• Function: chemokine activity; protein binding