Recombinant Rat CSF2 protein(Ala18-Lys144), His-tagged

• Cat.No.: CSF2-294R
• Product Overview: Recombinant Rat CSF2 (P48750) (Ala18-Lys144) was expressed in HEK293 with a polyhistidine tag at the N-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 18-144 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.02-0.1 ng/mL.
• Molecular Mass: The recombinant rat CSF2 comprises 147 amino acids and has a predicted molecular mass of 16.9 kDa. The apparent molecular mass of the protein is approximately 23 and 20 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Csf2 colony stimulating factor 2 (granulocyte-macrophage) [ Rattus norvegicus ]
• Official Symbol: CSF2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; CSF; colony-stimulating factor; Gmcsf; Gm-csf
• Gene ID: 116630