Recombinant Rat Cyp1a1 protein, His & GST-tagged

• Cat.No.: Cyp1a1-7964R
• Product Overview: Recombinant Rat Cyp1a1 aa. (Ser251~His521) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Monooxygenase that plays a role in dioxin metabolism and detoxification which is 3-methylcholanthrene-inducible.
• Source: E. coli
• Species: Rat
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser251~His521
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Cyp1a1 cytochrome P450, family 1, subfamily a, polypeptide 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp1a1
• Synonyms: Cyp1a1; cytochrome P450, family 1, subfamily a, polypeptide 1; AHH; AHRR; CP11; CYP1; Cyp45c; P1-450; P450-C; P450DX; Cypc45c; P-450MC; cytochrome P450 1A1; CYPIA1; P450MT2; P450form6; aryl hydrocarbon hydroxylase; cytochrome P1-450; cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c); cytochrome P450, 1a1; cytochrome P450, subfamily I (aromatic compound-inducible), member A1 (C6, form c) (C6, form c); cytochrome P450-C; cytochrome P450MT2; dioxin-inducible; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
• Gene ID: 24296
• mRNA Refseq: NM_012540.2
• Protein Refseq: NP_036672.2
• UniProt ID: P00185