Recombinant Rat DPEP1 Protein

• Cat.No.: DPEP1-1936R
• Product Overview: Recombinant Rat DPEP1 full length or partial length protein was expressed.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Dpep1 dipeptidase 1 (renal) [ Rattus norvegicus ]
• Official Symbol: DPEP1
• Gene ID: 94199
• mRNA Refseq: NM_053591.2
• Protein Refseq: NP_446043.1
• UniProt ID: P31430

Citation

Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver.

Journal: Cell    PubMed ID: 31442408    Data: 2020/5/11

Authors: Saurav Roy Choudhury, Liane Babes, Donna L Senger

Article Snippet:Agarose beads were quenched with 1M Tris, washed and resuspended in a 2X volume of PBS.Agarose beads were quenched with 1M Tris, washed and resuspended in a 2X volume of PBS.. 2 mg of C-terminal His-tagged recombinant DPEP1 (Creative BioMart, DPEP1-77H), and 30 mL agarose beads linked to LSALT, scrambled LSALT, GFE1 or GFE2 peptide were incubated in 200 ml of binding buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.05% NP-40) for 4 hours at 4 C. For the competition assay, 2 mg of unconjugated LSALT peptide was added to the recombinant DPEP1 and LSALT-agarose beads mixture.. Beads were washed three times with binding buffer and boiled with 50 mL of 2X Laemmli buffer.Beads were washed three times with binding buffer and boiled with 50 mL of 2X Laemmli buffer.