Active Recombinant Rat Fgf13 protein, His-tagged

• Cat.No.: Fgf13-8156R
• Product Overview: Active Recombinant Rat Fgf13 protein(Q9ERW3)(1-192 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 1-192 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: FGF13 (Fibroblast growth factor 13) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant rat FGF13 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF13. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF13 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF13 for 48h. The result was shown. It was obvious that FGF13 significantly increased cell viability of 3T3 cells.
• Molecular Mass: 22.9kDa
• Purity: > 97%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Fgf13 fibroblast growth factor 13 [ Rattus norvegicus ]
• Official Symbol: Fgf13
• Synonyms: FGF13; fibroblast growth factor 13
• Gene ID: 84488
• mRNA Refseq: NM_053428
• Protein Refseq: NP_445880