Recombinant Rat Gdnf protein, His-tagged

• Cat.No.: Gdnf-780R
• Product Overview: Recombinant Rat Gdnf aa. (Ser78~Ile211 (Accession # Q07731)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Neurotrophic factor specific for midbrain dopamine neurons; binds to GDNFR-alpha and mediates activation of the Ret protein tyrosine kinase receptor.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.2kDa
• Protein length: Ser78~Ile211 (Accession # Q07731)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Gdnf glial cell derived neurotrophic factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gdnf
• Synonyms: Gdnf; glial cell derived neurotrophic factor; gndf; glial cell line-derived neurotrophic factor; ATF; Glial cell line derived neutrophic factor; astrocyte-derived trophic factor
• Gene ID: 25453
• mRNA Refseq: NM_019139.1
• Protein Refseq: NP_062012.1
• UniProt ID: Q07731