Recombinant Rat Gusb protein, His-tagged

• Cat.No.: Gusb-6930R
• Product Overview: Recombinant Rat Gusb aa. (Val440~Phe648) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Catalyzes the conversion of a beta-D-glucuronoside and H2O to an alcohol and D-glucuronate.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Val440~Phe648
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gusb glucuronidase, beta [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gusb
• Synonyms: Gusb; glucuronidase, beta; Gus-s; Ac2-223; beta-glucuronidase
• Gene ID: 24434
• mRNA Refseq: NM_017015.2
• Protein Refseq: NP_058711.2
• UniProt ID: P06760