Recombinant Rat Hgf protein, His-tagged

• Cat.No.: Hgf-795R
• Product Overview: Recombinant Rat Hgf aa. (Cys306~Glu471) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Cys306~Glu471
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 6.8.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Hgf hepatocyte growth factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Hgf
• Synonyms: Hgf; hepatocyte growth factor; HPTA; hepatocyte growth factor; SF; hepatopoeitin-A; hepatopoietin-A; scatter factor
• Gene ID: 24446
• mRNA Refseq: NM_017017.2
• Protein Refseq: NP_058713.1
• UniProt ID: P17945