Active Recombinant Rat Ifng protein, His-tagged

• Cat.No.: Ifng-549R
• Product Overview: Active Recombinant Rat Ifng protein(P01581)(23~156 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 23-156 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA. Effect of IFN-γ on morphological change of Raw 246.7 cells was shown.
• Molecular Mass: 16.8kDa
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ifng interferon gamma [ Rattus norvegicus ]
• Official Symbol: Ifng
• Synonyms: IFNG; interferon gamma; IFN-gamma; IFNG2
• Gene ID: 25712
• mRNA Refseq: NM_138880
• Protein Refseq: NP_620235