Recombinant Rat Il13 Protein, His-tagged

• Cat.No.: Il13-1661R
• Product Overview: Recombinant Rat Il13 Protein (Pro22-His131) with N-His tag was expressed in E. coli.

Specification

• Description: Enables interleukin-13 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of cation transmembrane transport. Located in extracellular space. Used to study asthma; extrinsic allergic alveolitis; and lipoid nephrosis. Biomarker of several diseases, including alcoholic hepatitis; middle cerebral artery infarction; peptic esophagitis; prostatic hypertrophy; and pulmonary fibrosis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 15.8 kDa
  Accurate Molecular Mass: 16 kDa
• Protein length: Pro22-His131
• Purity: > 80%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Il13 interleukin 13 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il13
• Synonyms: IL13; interleukin 13; interleukin-13; IL-13; T-cell activation protein P600
• Gene ID: 116553
• mRNA Refseq: NM_053828
• Protein Refseq: NP_446280
• UniProt ID: P42203

Reviews

11/29/2021

Effective in Th2 cell differentiation.

04/22/2020

Good for cytokine assays.

01/26/2019

High yield in expression.

Q&As

How does flow cytometry help quantify IL13 receptor expression on different cell populations?

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions?

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures?

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes?

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes?

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways?

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity?

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes?

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes?

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding?

Site-directed mutagenesis identifies key residues in IL13 receptor binding.