Recombinant Rat IL2RG cell lysate

Cat.No. : IL2RG-1476RCL
Product Overview : Rat IL2RG / CD132 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Rat
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Il2rg interleukin 2 receptor, gamma [ Rattus norvegicus ]
Official Symbol : IL2RG
Synonyms : IL2RG; interleukin 2 receptor, gamma; cytokine receptor common subunit gamma; interleukin 2 receptor, gamma (severe combined immunodeficiency); Cd132; Ab2-183;
Gene ID : 140924
mRNA Refseq : NM_080889
Protein Refseq : NP_543165
Pathway : Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem;
Function : cytokine binding; cytokine receptor activity; interleukin-2 binding; interleukin-2 binding; interleukin-7 binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Are there any side effects of using this protein? 12/23/2022

The use of IL2RG may cause chest tightness, skin itching, allergies and other side effects, you need to contact the doctor for timely treatment.

What is the dosage and use method of IL2RG? 11/03/2022

The dosage and use method of IL2RG need to be determined according to the condition and individual situation, and need to be prescribed by a clinician.

Does IL2RG have a role and what is the mechanism? 11/28/2021

Enhances the immunity of the human immune system by increasing the number and improving the function of immune cells in the body.

Which diseases is IL2RG used to treat? 06/22/2021

IL2RG is mainly used in the treatment of immune deficiency diseases, such as systemic eosinophilic granulomatosis, X-linked immunodeficiency disease, etc.

What measures can be taken to mitigate the possible risks of IL2RG? 06/15/2021

In view of possible risks, the use of IL2RG should be strictly in accordance with the doctor's advice, and avoid changing the dosage and frequency of medication.

What are the pharmacological characteristics of IL2RG? 08/14/2019

IL2RG pharmacological features are contained in vivo to enhance the immunity of the human immune system by increasing the number of immune cells and improving their function.

Customer Reviews (3)

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Reviews
10/13/2022

    Solubility is very good and can be easily dissolved in various buffers.

    05/13/2022

      Quality I use is very good and I have recommended this product to my colleagues and friends.

      05/16/2021

        The anti-pollution ability is very strong, not easy to be polluted.

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