Active Recombinant Rat Il3 protein, His-tagged

Name: Active Recombinant Rat Il3 protein, His-tagged
SKU: Il3-1037R

Species :	Rat
Source :	HEK293
Tag :	His
Protein Length :	27-166 aa
Form :	Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity :	Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor. IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. IL-3 can also support the growth and survival of myeloid progenitor cells through the activation of Janus Kinase 2 (JAK2) tyrosine kinase, and macrophage differentiation has been shown to be regulated by protein kinase C (PKC). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat IL-3 and recombinant rat JAK2. Briefly, IL-3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL-3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450 nm immediately. The binding activity of IL-3 and JAK2 was shown, and this effect was in a dose dependent manner.
Molecular Mass :	17.4kDa
Endotoxin :	<1.0EU per 1μg (determined by the LAL method).
Purity :	> 95% as determined by SDS-PAGE.
Storage :	Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution :	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Name	Il3 interleukin 3 [ Rattus norvegicus ]
Official Symbol	Il3
Synonyms	IL3; interleukin 3; interleukin-3; IL-3; MCGF; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor;
Gene ID	24495
mRNA Refseq	NM_031513
Protein Refseq	NP_113701