Active Recombinant Rat Il4 protein, His-GST-tagged

• Cat.No.: Il4-648R
• Product Overview: Active Recombinant Rat Il4 protein(P20096)(61~111 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: 61-111 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL4 and recombinant rat IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 35.7kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il4 interleukin 4 [ Rattus norvegicus ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor; Il4e12
• Gene ID: 287287
• mRNA Refseq: NM_201270
• Protein Refseq: NP_958427