Recombinant Rat IL9R cell lysate
|Product Overview :||Rat IL9R / Interleukin 9 receptor derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
- Gene Information
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|Source :||Human cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
|Gene Name :||Il9r interleukin 9 receptor [ Rattus norvegicus ]|
|Official Symbol :||IL9R|
|Synonyms :||IL9R; interleukin 9 receptor; interleukin-9 receptor;|
|Gene ID :||24500|
|mRNA Refseq :||NM_017021|
|Protein Refseq :||NP_058717|
|Pathway :||Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;|
|Function :||interleukin-9 binding; interleukin-9 receptor activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
In pre-clinical trials, the IL9R significantly increased natural killer and CD4 + T cell levels in patients.
IL9R may play an important role in the treatment of central nervous system diseases and hematological diseases.
This needs to be further confirmed by large-scale clinical trials.
This can be achieved through in vitro molecular cell techniques and carefully designed in vivo animal models.
There have been no large-scale clinical studies to prove which diseases the recombinant IL9R protein can treat.
Some studies have suggested that the expression of IL9R in tumor cells may be associated with patient prognosis.
Customer Reviews (3)Write a review
IL9R is very advanced and adopts the most environmentally friendly production process, which is worth vigorously promoting. -
The reproducibility of IL9R is very good, and the results are very consistent in each experiment or application, thanks to its high stability and reproducibility, which provides great convenience for follow-up studies. -
IL9R produced by them are widely used and suitable for various biological experiments. -
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