Recombinant Rat Insulin Degrading Enzyme, His-tagged

• Cat.No.: Ide-761R
• Product Overview: Recombinant rat insulin degrading enzyme was fused to a His•Tag sequence and expressed inS. frugiperdainsect cells.MW = 110 kDa.

Specification

• Species: Rat
• Source: S.frugiperda
• Tag: His
• Description: Insulin Degrading Enzyme (IDE) is a large zinc-binding protease of the M16A metalloprotease subfamily known to cleave multiple short polypeptides that vary considerably in sequence. IDE was first identified by its ability to degrade the B chain of the hormone insulin. This activity was observed over fifty years ago, though the enzyme specifically responsible for B chain cleavage was identified more recently. This discovery revealed considerable amino acid sequence homology between IDE and the previously characterized bacterial protease pitrilysin, suggesting a common proteolytic mechanism.
• Purity: ≥90% by SDS-PAGE.
• Form: Liquid. In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.
• Specific Activity: ≥3 U/mg protein.
• Unit Definition: One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.
• Specificity: ≥4 units/mg protein.
• Storage: ≤ -70℃. Avoid freeze/thaw.

Gene Information

• Gene Name: Ide insulin degrading enzyme [ Rattus norvegicus ]
• Synonyms: Ide; insulin degrading enzyme; INSDEGM; insulin-degrading enzyme; insulysin; insulinase; insulin protease; EC 3.4.24.56
• Gene ID: 25700
• mRNA Refseq: NM_013159
• Protein Refseq: NP_037291
• UniProt ID: P35559
• Chromosome Location: 1q53
• Pathway: Alzheimer"s disease
• Function: ATP binding; ATPase activity; beta-amyloid binding; beta-endorphin binding; insulin binding; metal ion binding; metalloendopeptidase activity; peptidase activity; nucleotide binding; peptide hormone binding; protein binding; protein homodimerization activity; zinc ion binding