Recombinant Rat Interleukin 4
Cat.No. : | Il4-96R |
Product Overview : | Recombinant Rat Interleukin-4 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 16 kDa. The IL-4 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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Cat. No. : | Il4-96R |
Description : | IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
Source : | Escherichia Coli. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys-Asn. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
Solubility : | It is recommended to reconstitute the lyophilized I-TAC in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Specific Activity : | The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 6.6 x 10⁵ IU/mg. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
Gene Name : | Il4 interleukin 4 [ Rattus norvegicus ] |
Synonyms : | Il4; interleukin 4; Il4e12 |
Gene ID : | 287287 |
mRNA Refseq : | NM_201270 |
Protein Refseq : | NP_958427 |
UniProt ID : | P20096 |
Chromosome Location : | 10q22 |
Pathway : | Allograft rejection; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; Jak-STAT signaling pathway; T cell receptor signaling pathway |
Function : | cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewBy testing immunogenicity in animal models, detect whether an immune response is induced.
The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.
This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.
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