Species : |
Rat |
Source : |
E.coli |
Tag : |
Non |
Description : |
Leptin (Greek leptos meaning thin) is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. The Lep is located on chromosome 7 in humans. |
Form : |
Filtered (0, 4 μm) and lyophilized in 0.5 mg/mL in 0.002 mM NaHCO3 |
Research topic : |
Energy metabolism and body weight regulation |
Purity : |
>95% |
Reconstitution : |
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
Applications : |
ELISA; Western blotting |
Quality Control Test : |
BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein |
Sequences of amino acids : |
VPIHKVQDDT KTLIKTIVTR INDISHTQSV SARQRVTGLD FIPGLHPILS LSKMDQTLAV YQQILTSLPS QNVLQIAHDL ENLRDLLHLL AFSKSCSLPQ TRGLQKPESL DGVLEASLYS TEVVALSRLQ GSLQDILQQL DLSPEC |
Storage : |
Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C. |