Recombinant Rat MAPT Protein, His-tagged

• Cat.No.: MAPT-648R
• Product Overview: Recombinant Rat MAPT Protein(NP_058908)(2-752aa), fused to N-terminal His tag, was expressed in Yeast.

Specification

• Source: Yeast
• Species: Rat
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 80.4kDa
• Protein length: 2-752aa
• AA Sequence: AEPRQEFDTMEDQAGDYTMLQDQEG DMDHGLKESPPQPPADDGSEEPGSE TSDAKSTPTAEDVTAPLVEERAPDK QATAQSHTEIPEGTTAEEAGIGDTP NMEDQAAGHVTQEPQKVEIFSQSLL VEPGRREGQAPDSGISDWTHQQVPS MSGAPLPPQGLREATHQPLGTRPED VERSHPASELLWQESPQKEAWGKDR LGSEEEVDEDITMDESSQESPPSQA SLAPGTATPQARSVSASGVSGETTS IPGFPAEGSIPLPADFFSKVSAETQ ASPPEGPGTGPSEEGHEAAPEFTFH VEIKASAPKEQDLEGATVVGAPAEE QKARGPSVGKGTKEASLLEPTDKQP AAGLPGRPVSRVPQLKARVAGVSKD RTGNDEKKAKTSTPSCAKTPSNRPC LSPTRPTPGSSDPLIKPSSPAVCPE PATSPKYVSSVTPRNGSPGTKQMKL KGADGKTGAKIATPRGAATPGQKGT SNATRIPAKTTPSPKTPPGSGEPPK SGERSGYSSPGSPGTPGSRSRTPSL PTPPTREPKKVAVVRTPPKSPSASK SRLQTAPVPMPDLKNVRSKIGSTEN LKHQPGGGKVQIINKKLDLSNVQSK CGSKDNIKHVPGGGSVHIVYKPVDL SKVTSKCGSLGNIHHKPGGGQVEVK SEKLDFKDRVQSKIGSLDNITHVPG GGNKKIETHKLTFRENAKAKTDHGA EIVYKSPVVSGDTSPRHLSNVSSTG SIDMVDSPQLATLADEVSASLAKQG L
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: Mapt microtubule-associated protein tau [ Rattus norvegicus ]
• Official Symbol: MAPT
• Synonyms: MAPT; microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; Tau microtubule-associated protein; pTau; Mtapt; RNPTAU; MGC156663
• Gene ID: 29477
• mRNA Refseq: NM_017212
• Protein Refseq: NP_058908
• UniProt ID: P19332

Reviews

07/22/2022

Western-blot experiments using MAPT as a target have shown highly reproducible results.

08/08/2020

This stability of MAPT is very good and can maintain its original properties even after multiple uses.

09/15/2019

MAPT's purity and consistency make it a top choice for Western-blot experiments.

Q&As

How to assess the degree of abnormal aggregation of MAPT protein?

The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

What is the relationship between MAPT protein and nerve cytoskeleton?

The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

What are the interactions of the MAPT protein with other proteins in the nervous system?

MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

Is there a mechanism that regulates the expression of MAPT protein?

Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

What are the future directions for MAPT protein research?

Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

How about the applications of MAPT protein in clinical diagnosis?

The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.