Recombinant Rat Mmp3 protein, His-tagged

• Cat.No.: Mmp3-745R
• Product Overview: Recombinant Rat Mmp3 aa. (Asn278~Gly450) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions
• Protein length: Asn278~Gly450
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Publication:
  Loss of TIMP3 expression induces inflammation, matrix degradation, and vascular ingrowth in nucleus pulposus: A new mechanism of intervertebral disc degeneration (2020)

Gene Information

• Gene Name: Mmp3 matrix metallopeptidase 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mmp3
• Synonyms: MMP-3; PTR1 protein; SL-1; matrix metalloproteinase-3; transin-1; stromelysin-1
• Gene ID: 171045
• mRNA Refseq: NM_133523.3
• Protein Refseq: NP_598207.2

Reviews

12/05/2020

Critical expertise in protein engineering, a trusted partner in research.

03/21/2020

Swift and reliable protein sequencing, a key asset for our work.

01/22/2020

High-quality protein crystallography services, consistently delivers exceptional outcomes.

Q&As

How does MMP3 (Matrix Metalloproteinase 3) facilitate extracellular matrix remodeling?

MMP3 breaks down extracellular matrix components, aiding in tissue remodeling and repair.

What is the impact of MMP3 on cancer metastasis and tumor microenvironment modulation?

MMP3 facilitates cancer metastasis by altering the tumor microenvironment and promoting invasion.

How do alterations in MMP3 expression or activity affect skin aging and the development of dermatological conditions?

Changes in MMP3 activity can accelerate skin aging and contribute to dermatological conditions.

How does MMP3 influence cardiovascular diseases, particularly in atherosclerosis and aneurysm formation?

MMP3 influences cardiovascular health by affecting arterial wall remodeling and stability.

What role does MMP3 play in the progression of arthritis and joint degeneration?

MMP3 contributes to arthritis progression by degrading joint cartilage and connective tissues.

What are the mechanisms by which MMP3 modulates inflammatory responses?

MMP3 modulates inflammation, impacting tissue inflammation and repair processes.

How does MMP3 contribute to wound healing and tissue repair processes?

MMP3 plays a role in wound healing by remodeling damaged tissues and aiding in repair.