Recombinant Rat Mmp3 protein, His-tagged

• Cat.No.: Mmp3-745R
• Product Overview: Recombinant Rat Mmp3 aa. (Asn278~Gly450) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions
• Protein length: Asn278~Gly450
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Publication:
  Loss of TIMP3 expression induces inflammation, matrix degradation, and vascular ingrowth in nucleus pulposus: A new mechanism of intervertebral disc degeneration (2020)

Gene Information

• Gene Name: Mmp3 matrix metallopeptidase 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mmp3
• Synonyms: MMP-3; PTR1 protein; SL-1; matrix metalloproteinase-3; transin-1; stromelysin-1
• Gene ID: 171045
• mRNA Refseq: NM_133523.3
• Protein Refseq: NP_598207.2