Recombinant Rat Pdcd1 Protein, hFc-tagged, Alexa Fluor 555 conjugated
Cat.No. : | Pdcd1-560RAF555 |
Product Overview : | Alexa Fluor 555 conjugated recombinant Rat Pdcd1 (Leu25-Gln167) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293). |
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Description : | Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. |
Source : | HEK293 |
Species : | Rat |
Tag : | hFc |
Form : | Lyophilized |
Molecular Mass : | The protein has a calculated MW of 42.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE). |
Protein length : | Leu25-Gln167 |
N-terminal Sequence Analysis : | Leu 25 |
Endotoxin : | < 1.0 EU/ μg by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | Pdcd1 |
Official Symbol : | Pdcd1 |
Synonyms : | PDCD1; programmed cell death 1; programmed cell death protein 1 |
Gene ID : | 301626 |
mRNA Refseq : | NM_001106927 |
Protein Refseq : | NP_001100397 |
UniProt ID : | D3ZIN8 |
Products Types
◆ Recombinant Protein | ||
PDCD1-266H | Recombinant Human PDCD1 Protein (ECD), Fc-His-tagged(C-ter) | +Inquiry |
Pdcd1-823MB | Active BiotinylatedRecombinant Mouse Pdcd1 protein(Met1-Gln167), His-tagged | +Inquiry |
PDCD1-15H | Recombinant Human PDCD1 Protein, C-6His-Avi tagged, Biotinylated | +Inquiry |
PDCD1-1174C | Recombinant Canine PDCD1 protein(Met1-Gly168), His&Avi-tagged, Biotinylated | +Inquiry |
PDCD1-701R | Recombinant Rabbit PDCD1 Protein | +Inquiry |
◆ Lysates | ||
PDCD1-2628MCL | Recombinant Mouse PDCD1 cell lysate | +Inquiry |
PDCD1-001RCL | Recombinant Rat PDCD1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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