Recombinant Rat protein
Cat.No. : | Vegfa-7862R |
Product Overview : | Recombinant Rat Vegfa protein (165 a.a.) was expressed in Yeast. |
- Specification
- Gene Information
- Related Products
Description : | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. |
Source : | Yeast |
Species : | Rat |
Form : | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75‑3.75 ng/mL. |
Molecular Mass : | Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions. |
Protein length : | 165 |
AA Sequence : | MAPTTEGEQKAHEVVKFMDVYQRSY CRPIETLVDIFQEYPDEIEYIFKPS CVPLMRCAGCCNDEALECVPTSESN VTMQIMRIKPHQSQHIGEMSFLQHS RCECRPKKDRTKPEKHCEPCSERRK HLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR |
Endotoxin : | Less than 0.1 EU/μg of rRtVEGF164, Yeast as determined by LAL method. |
Purity : | >95% by SDS-PAGE and 90% |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Vegfa |
Official Symbol : | Vegfa |
Synonyms : | VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164; |
Gene ID : | 83785 |
mRNA Refseq : | NM_001110333 |
Protein Refseq : | NP_001103803 |
UniProt ID : | P16612 |
Products Types
◆ Recombinant Protein | ||
Vegfa-878R | Active Recombinant Rat Vegfa protein(Met1-Arg190) | +Inquiry |
VEGFA-292H | Active Recombinant Human VEGFA Protein (Ala27-Arg191), C-His tagged, Animal-free, Carrier-free | +Inquiry |
Vegfa-475M | Active Recombinant Mouse Vegfa protein(Met1-Arg190) | +Inquiry |
VEGFA-700H | Recombinant Human VEGFA Protein (Met1-Arg136) | +Inquiry |
Vegfa-145V | Active Recombinant Rat Vegf164 Protein (165 aa) | +Inquiry |
◆ Native Protein | ||
VEGFA-31701TH | Native Human VEGFA | +Inquiry |
◆ Lysates | ||
VEGFA-655HCL | Recombinant Human VEGFA cell lysate | +Inquiry |
VEGFA-647MCL | Recombinant Mouse VEGFA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionStudies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.
At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.
VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.
The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.
Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.
VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.
Customer Reviews (2)
Write a reviewVEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.
VEGFA they produced was highly active and the experimental effect was obvious.
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