Active Recombinant Rat protein

Name: Active Recombinant Rat protein
SKU: Vegfa-7862R

Specification
Gene Information
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Species :	Rat
Source :	Yeast
Tag :	Non
Protein Length :	27-190 aa
Form :	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity :	Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75‑3.75 ng/mL.
Molecular Mass :	Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions.
AASequence :	MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Endotoxin :	Less than 0.1 EU/μg of rRtVEGF164, Yeast as determined by LAL method.
Purity :	>95% by SDS-PAGE and 90%
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
Official Symbol	Vegfa
Synonyms	VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164;
Gene ID	83785
mRNA Refseq	NM_001110333
Protein Refseq	NP_001103803