Recombinant Rat Rpp40 Protein, His-tagged

• Cat.No.: Rpp40-2034R
• Product Overview: Recombinant Rat Rpp40 Protein (Met91-Asp259) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable ribonuclease P RNA binding activity. Predicted to contribute to ribonuclease MRP activity and ribonuclease P activity. Predicted to be involved in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); endonucleolytic cleavage involved in tRNA processing; and tRNA 5'-leader removal. Predicted to be located in nucleolus. Predicted to be part of multimeric ribonuclease P complex and ribonuclease MRP complex. Orthologous to human RPP40 (ribonuclease P/MRP subunit p40).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.0 kDa
  Accurate Molecular Mass: 23 kDa
• Protein length: Met91-Asp259
• Purity: > 97%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Rpp40 ribonuclease P/MRP subunit p40 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Rpp40
• Synonyms: Rpp40; ribonuclease P/MRP subunit p40; ribonuclease P protein subunit p40; RNaseP protein p40; ribonuclease P 40 subunit; ribonuclease P/MRP 40 subunit; EC 3.1.26.5
• Gene ID: 291071
• mRNA Refseq: NM_001013055
• Protein Refseq: NP_001013073
• UniProt ID: Q5BK64