| Species : |
Rat |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
Scgn isa protein that in humans is encoded by the Scgn gene. The encoded protein isa secreted calcium-binding protein which is found in the cytoplasm. It isrelated to calbindin D-28K and calretinin. This protein is thought to beinvolved in KCL-stimulated calcium flux and cell proliferation. |
| Form : |
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mMTris buffer, 50mM NaCl, pH 7.5. |
| Researchtopic : |
Diabetology-Autoimmunity; Neural tissue damage markers;Oncology; Pancreatic regulatory molecules. |
| Purity : |
>95% |
| Reconstitution : |
Adddeionized water to prepare a working stock solution of approximately 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile.Please filter the product by an appropriate sterile filter before using it inthe cell culture. |
| Applications : |
Western blotting |
| QualityControl Test : |
BCA to determine quantity of theprotein; SDS PAGE to determine purity of the protein. |
| Sequences of aminoacids : |
MKHHHHHHAS MDNAHRQTQA HLDAACFWQI WQRFDKDEKG YIKETELDAFFDDLLAKFGI EDTLMEENVQ KMKEQLMVGH DISKEGRILM KELASMFLSE DENFLLFFRL ETPLDNSVEFMQIWRKYDAD SSGFISAAEL SNFLRDLFLH HKKVISEAEL EEYTSTMMKI FDRNKDGRLD LNDLARILALQENFLLQFKM DASSTEERKR DFEKIFAHYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILLRHCDVNKDGK IQKSELALCL GLKINP |
| Storage : |
Store lyophilized protein at -20°C. Lyophilized protein remainsstable until the expiry date when stored at -20°C. Aliquot reconstitutedprotein to avoid repeated freezing/thawing cycles and store at -80°C for longterm storage. Reconstituted protein can be stored at 4°C for a limited periodof time; it does not show any change after one week at 4°C. |
