Recombinant Rat Serping1 Protein, His-tagged

• Cat.No.: Serping1-534R
• Product Overview: Recombinant Rat Serping1 (NP_954524.1) (Met1-Ala504) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Description: Plasma protease C1 inhibitor, also known as C1-inhibiting factor, C1-INH, C1 esterase inhibitor, SERPING1 and C1IN, is a serine proteinase inhibitor (serpin) that regulates activation of both the complement and contact systems. By its C-terminal part (serpin domain), characterized by three beta-sheets and an exposed mobile reactive loop, C1-INH binds, and bl centigradeks the activity of its target proteases. The N-terminal end (nonserpin domain) confers to C1-INH the capacity to bind lipopolysaccharides and E-selectin. Owing to this moiety, C1-INH intervenes in regulation of the inflammatory reaction. The heterozygous deficiency of C1-INH results in hereditary angioedema (HAE). Owing to its ability to modulate the contact and complement systems and the convincing safety profile, plasma-derived C1 inhibitor is an attractive therapeutic protein to treat inflammatory diseases other than HAE. Deficiency of C1 inhibitor results in hereditary angioedema, which is characterized by recurrent episodes of l centigradealized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa. C1 inhibitor may prove useful in a variety of other diseases including septic sh centigradek, reperfusion injury, hyperacute transplant rejection, traumatic and hemorrhagic sh centigradek, and the increased vascular permeability ass centigradeiated with thermal injury, interleukin-2 therapy and cardiopulmonary bypass.
• Source: HEK293
• Species: Rat
• Tag: His
• Predicted N Terminal: Asp 23
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat Serping1 consists of 493 amino acids and predicts a molecular mass of 54.7 kDa.
• Protein length: Met1-Ala504
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Serping1 serpin peptidase inhibitor, clade G, member 1 [ Rattus norvegicus ]
• Official Symbol: Serping1
• Synonyms: SERPING1; serpin peptidase inhibitor, clade G, member 1; plasma protease C1 inhibitor; C1Inh; C1 Inh; serpin G1; C1-inhibiting factor; C1 esterase inhibitor; serine (or cysteine) peptidase inhibitor, clade G, member 1; serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary); MGC72585
• Gene ID: 295703
• mRNA Refseq: NM_199093
• Protein Refseq: NP_954524