Recombinant Rat Spam1 Protein, His-tagged

• Cat.No.: Spam1-2091R
• Product Overview: Recombinant Rat Spam1 Protein (Val36-Cys464) with N-His tag was expressed in E. coli.

Specification

• Description: Predicted to enable hyalurononglucosaminidase activity. Predicted to be involved in hyaluronan catabolic process. Predicted to act upstream of or within single fertilization. Predicted to be located in external side of plasma membrane and membrane raft. Predicted to be active in acrosomal vesicle. Orthologous to human SPAM1 (sperm adhesion molecule 1).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 52.7 kDa
  Accurate Molecular Mass: 53 kDa
• Protein length: Val36-Cys464
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Spam1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Spam1
• Synonyms: SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); hyaluronidase PH-20; hyal-PH20; sperm surface antigen 2B1; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; Spam; MGC93268
• Gene ID: 117037
• mRNA Refseq: NM_053967
• Protein Refseq: NP_446419
• UniProt ID: Q62803