Recombinant Full Length Human SPAM1 Protein, C-Flag-tagged

• Cat.No.: SPAM1-868HFL
• Product Overview: Recombinant Full Length Human SPAM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 58.2 kDa
• AA Sequence: MGVLKFKHIFFRSFVKSSGVSQIVF TFLLIPCCLTLNFRAPPVIPNVPFL WAWNAPSEFCLGKFDEPLDM SLFSFIGSPRINATGQGVTIFYVDR LGYYPYIDSITGVTVNGGIPQKISL QDHLDKAKKDITFYMPVDNL GMAVIDWEEWRPTWARNWKPKDVYK NRSIELVQQQNVQLSLTEATEKAKQ EFEKAGKDFLVETIKLGKLL RPNHLWGYYLFPDCYNHHYKKPGYN GSCFNVEIKRNDDLSWLWNESTALY PSIYLNTQQSPVAATLYVRN RVREAIRVSKIPDAKSPLPVFAYTR IVFTDQVLKFLSQDELVYTFGETVA LGASGIVIWGTLSIMRSMKS CLLLDNYMETILNPYIINVTLAAKM CSQVLCQEQGVCIRKNWNSSDYLHL NPDNFAIQLEKGGKFTVRGK PTLEDLEQFSEKFYCSCYSTLSCKE KADVKDTDAVDVCIADGVCIDAFLK PPMETEEPQIFYNASPSTLS
  ATMFIWRLEVWDQGISRIGFFTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways

Gene Information

• Gene Name: SPAM1 sperm adhesion molecule 1 [ Homo sapiens (human) ]
• Official Symbol: SPAM1
• Synonyms: HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• Gene ID: 6677
• mRNA Refseq: NM_003117.5
• Protein Refseq: NP_003108.2
• MIM: 600930
• UniProt ID: P38567