Active Recombinant Rat Tgfb1 protein, His-tagged

• Cat.No.: Tgfb1-531R
• Product Overview: Active Recombinant Rat Tgfb1 protein(P17246)(279~390 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 279-390 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Transforming Growth Factor Beta 1 (TGFb1) is a multifunctional set of peptidesthat controls proliferation, differentiation, and other functions in manycell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Besides, Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) has been identified as an interactor of TGFb1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TGFb1 and recombinant mouse LTBP1 (high homology with rat). Briefly, TGFb1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uLwere then transferred to LTBP1-coated microtiter wells and incubatedfor 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TGFb1pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGFb1 and LTBP1 was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 14.1kDa
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Rattus norvegicus ]
• Official Symbol: Tgfb1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta-1; transforming growth factor, beta-1; Tgfb
• Gene ID: 59086
• mRNA Refseq: NM_021578
• Protein Refseq: NP_067589