Recombinant Rat Tnfrsf18 Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Tnfrsf18-8735RAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Rat Tnfrsf18 (Gln20-Lys121) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Gln20-Lys121
• Description: Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 37.2 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• N-terminal Sequence Analysis: Gln 20
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 488

Gene Information

• Gene Name: Tnfrsf18
• Official Symbol: Tnfrsf18
• Synonyms: TNFRSF18; tumor necrosis factor receptor superfamily, member 18; tumor necrosis factor receptor superfamily member 18
• Gene ID: 500598
• mRNA Refseq: NM_001024349
• Protein Refseq: NP_001019520
• UniProt ID: F7FFS7