Recombinant Rat Tpbg Protein, His-tagged

• Cat.No.: Tpbg-11162R
• Product Overview: Rat TPBG Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 32 - Ser 361 (Accession # Q5PQV5). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Ser 32

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 32-361 aa
• Description: Trophoblast glycoprotein (TPBG), also known as 5T4, is the therapeutic target of several anticancer agents currently in clinical development, largely due to its high expression in tumors and low expression in normal adult tissues.This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: The protein has a calculated MW of 37.7 kDa. The protein migrates as 50-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Tpbg trophoblast glycoprotein [ Rattus norvegicus ]
• Official Symbol: TPBG
• Synonyms: TPBG; trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4 oncofetal trophoblast glycoprotein; 5T4; MGC93332
• Gene ID: 83684
• mRNA Refseq: NM_031807
• Protein Refseq: NP_113995
• UniProt ID: Q5PQV5