Recombinant Rhesus macaque B7H5 protein, His-tagged

• Cat.No.: B7H5-346H
• Product Overview: Recombinant Rhesus macaque B7H5 protein (Phe33-Thr192), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 160
• Description: Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 19.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VSIR
• Official Symbol: VSIR
• Synonyms: C9orf54; C9H10orf54; B7-H5; SISP1; Gi24; VISTA
• Gene ID: 709595
• mRNA Refseq: NM_001190878.1
• Protein Refseq: NP_001177807.1
• UniProt ID: F7F6S6