|Product Overview:||Rhesus macaque VEGF R2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 764 (Accession # F7E313).|
|Description:||Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.|
|Predicted N Terminal:||Ala 20|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass:||This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 85.2 kDa. The protein migrates as 120-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length:||Ala 20 - Glu 764|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>97% as determined by SDS-PAGE.|
|Storage:||For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Gene Name:||KDR kinase insert domain receptor [ Macaca mulatta (Rhesus monkey) ]|
|Synonyms:||FLK-1; kinase insert domain receptor (a type III receptor tyrosine kinase)
; vascular endothelial growth factor receptor 2