Active Recombinant Rhesus monkey CXCL8 protein, His-tagged

• Cat.No.: CXCL8-667R
• Product Overview: Active Recombinant Rhesus monkey CXCL8 protein(P67813)(23~101 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Rhesus monkey
• Source: E.coli
• Tag: His
• Protein Length: 23-101 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells.Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of IL8 on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100ul cell suspension,10^6 cells/ml in RPMI 1640 with FBS free) and recombinant Simian IL8 (1ng/ml, 10ng/ml,100ng/ml and 1000ng/ml diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter(8um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 90min, the filter was removed,then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification(×400) randomly (five fields for each filter). Result shows IL8 is able to induce migration of Jurkat cells. The migrated Jurkat cells in low chamber at low magnification(×100) were shown. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification(×400). The optimum chemotaxis of IL8 occurs at 10-100ng/ml.
• Molecular Mass: 12.5kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.