Recombinant Saccharomyces Cerevisiae AGA2 Protein (19-87 aa), His-tagged
Cat.No. : | AGA2-1592S |
Product Overview : | Recombinant Saccharomyces Cerevisiae (strain ATCC 204508/S288c) AGA2 Protein (19-87 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein. |
- Specification
- Gene Information
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Description : | Receptor binding subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complentary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating. |
Source : | Yeast |
Species : | Saccharomyces Cerevisiae |
Tag : | His |
Form : | Tris-based buffer,50% glycerol |
Molecular Mass : | 9.5 kDa |
Protein length : | 19-87 aa |
AA Sequence : | QELTTICEQIPSPTLESTPYSLSTT TILANGKAMQGVFEYYKSVTFVSNC GSHPSTTSKGSPINTQYVF |
Purity : | > 90% as determined by SDS-PAGE. |
Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
Concentration : | A hardcopy of COA with reconstitution instruction is sent along with the products. |
Synonyms : | AGA2; |
UniProt ID : | P32781 |
Synonyms : | AGA2; |
UniProt ID : | P32781 |
Products Types
◆ Recombinant Protein | ||
AGA2-915S | Recombinant Saccharomyces Cerevisiae AGA2 Protein (19-87 aa), His-SUMO-tagged | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (20)
Ask a questionZinc ions slightly enhance Aga2's activity, while manganese ions inhibit its activity, even at low concentrations.
The research focuses on the enzymatic breakdown of seaweed-derived agar, driven by advancements in green technologies for utilizing marine biomass.
Agarases are enzymes that catalyze the breakdown of glycosidic bonds within polysaccharides, particularly agar. They play a crucial role in breaking down agar into smaller molecules.
The newly identified β-agarase is called Aga2.
Techniques such as protein-protein interaction studies, microscopy, and genetic manipulation are commonly used to study AGA2 and its role in yeast mating.
Aga2 is exclusive to the Cellulophaga genus, which means it is found only within this specific group of microorganisms.
AGA2 interacts with another protein called AGA1, forming a complex that allows yeast cells to adhere to each other during mating.
Bioinformatic analysis suggests that intrinsic properties of the protein, such as certain dipeptides and the relative volume of aliphatic amino acids, as well as tertiary structural elements like salt bridges,
AGA2 participates in the mating process of yeast cells, aiding in the binding and fusion of cells during sexual reproduction.
The AGA2 protein is involved in cell adhesion, particularly in yeast cells during mating, where it mediates the attachment of haploid cells to form diploid cells.
Aga2 belongs to the glycoside hydrolase 16 family.
Aga2 is an endolytic-type β-agarase, which means it cleaves glycosidic bonds within the agar molecule at internal positions.
The Km (Michaelis constant) for Aga2 is 2.59 mg/mL, the Vmax (maximum velocity) is 275.48 U/mg, the Kcat (catalytic rate constant) is 1.73 × 10^2 s^-1, and the Kcat/Km (efficiency constant) is 8.04 × 10^6 s^-1M^-1.
The AGA2 protein is found in yeast, specifically in Saccharomyces cerevisiae, a common model organism in biological research.
The Km (Michaelis constant) for Aga2 is 2.59 mg/mL, the Vmax (maximum velocity) is 275.48 U/mg, the Kcat (catalytic rate constant) is 1.73 × 10^2 s^-1, and the Kcat/Km (efficiency constant) is 8.04 × 10^6
The AGA2 protein contains domains that facilitate cell adhesion and interaction with its partner protein AGA1.
The expression of the AGA2 gene is influenced by the presence of mating pheromones, which trigger the mating response in yeast cells.
Aga2 exhibits optimum activity at a temperature of 45°C and a pH of 8.0.
Aga2 exhibits good thermostability at temperatures of 45°C and higher, and it retains over 90% of its activity even after undergoing repeated freeze-thaw cycles.
Customer Reviews (5)
Write a reviewProtein demonstrated purity levels suitable for sensitive assays
Dependable protein-protein interaction outcomes were enabled by the product’s pure composition.
It enabled clear visualization of cellular structures under the microscope.
The consistent performance of this protein simplified data interpretation.
We attributed the minimal background noise in our assays to the product’s superior quality.
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