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Recombinant Saccharomyces Cerevisiae AGA2 Protein (19-87 aa), His-tagged

Cat.No. : AGA2-1592S
Product Overview : Recombinant Saccharomyces Cerevisiae (strain ATCC 204508/S288c) AGA2 Protein (19-87 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
  • Specification
  • Gene Information
  • Related Products
Description : Receptor binding subunit of the a-agglutinin heterodimer. S.cerevisiae a and alpha cells express the complentary cell surface glycoproteins a-agglutinin and alpha-agglutinin, respectively, which interact with one another to promote cellular aggregation during mating.
Source : Yeast
Species : Saccharomyces Cerevisiae
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 9.5 kDa
Protein length : 19-87 aa
AA Sequence : QELTTICEQIPSPTLESTPYSLSTT TILANGKAMQGVFEYYKSVTFVSNC GSHPSTTSKGSPINTQYVF
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Synonyms : AGA2;
UniProt ID : P32781
Synonyms : AGA2;
UniProt ID : P32781

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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How do zinc and manganese ions affect Aga2's activity? 04/12/2022

Zinc ions slightly enhance Aga2's activity, while manganese ions inhibit its activity, even at low concentrations.

What is the focus of the recent research mentioned in the passage? 11/29/2021

The research focuses on the enzymatic breakdown of seaweed-derived agar, driven by advancements in green technologies for utilizing marine biomass.

What is the role of agarases in this context? 10/26/2021

Agarases are enzymes that catalyze the breakdown of glycosidic bonds within polysaccharides, particularly agar. They play a crucial role in breaking down agar into smaller molecules.

What is the name of the newly identified β-agarase? 10/02/2021

The newly identified β-agarase is called Aga2.

What techniques are commonly used to study the AGA2 protein's properties and behavior? 09/02/2021

Techniques such as protein-protein interaction studies, microscopy, and genetic manipulation are commonly used to study AGA2 and its role in yeast mating.

What is unique about the distribution of Aga2? 09/01/2021

Aga2 is exclusive to the Cellulophaga genus, which means it is found only within this specific group of microorganisms.

Does the AGA2 protein interact with other proteins in the cell? If so, which ones? 03/05/2021

AGA2 interacts with another protein called AGA1, forming a complex that allows yeast cells to adhere to each other during mating.

What factors contribute to Aga2's thermostability according to bioinformatic analysis? 02/09/2021

Bioinformatic analysis suggests that intrinsic properties of the protein, such as certain dipeptides and the relative volume of aliphatic amino acids, as well as tertiary structural elements like salt bridges,

What cellular processes or pathways does the AGA2 protein participate in? 12/20/2020

AGA2 participates in the mating process of yeast cells, aiding in the binding and fusion of cells during sexual reproduction.

What is the function of the AGA2 protein? 06/26/2020

The AGA2 protein is involved in cell adhesion, particularly in yeast cells during mating, where it mediates the attachment of haploid cells to form diploid cells.

To which family of enzymes does Aga2 belong? 02/08/2020

Aga2 belongs to the glycoside hydrolase 16 family.

What type of agarase activity does Aga2 exhibit? 01/28/2019

Aga2 is an endolytic-type β-agarase, which means it cleaves glycosidic bonds within the agar molecule at internal positions.

What are the kinetic parameters of Aga2? 12/01/2018

The Km (Michaelis constant) for Aga2 is 2.59 mg/mL, the Vmax (maximum velocity) is 275.48 U/mg, the Kcat (catalytic rate constant) is 1.73 × 10^2 s^-1, and the Kcat/Km (efficiency constant) is 8.04 × 10^6 s^-1M^-1.

In which organisms is the AGA2 protein found? 11/17/2018

The AGA2 protein is found in yeast, specifically in Saccharomyces cerevisiae, a common model organism in biological research.

What are the kinetic parameters of Aga2? 11/10/2018

The Km (Michaelis constant) for Aga2 is 2.59 mg/mL, the Vmax (maximum velocity) is 275.48 U/mg, the Kcat (catalytic rate constant) is 1.73 × 10^2 s^-1, and the Kcat/Km (efficiency constant) is 8.04 × 10^6

How is the AGA2 protein's structure related to its function? 06/29/2018

The AGA2 protein contains domains that facilitate cell adhesion and interaction with its partner protein AGA1.

Is the expression of the AGA2 gene regulated? If yes, what factors control its expression? 02/15/2018

The expression of the AGA2 gene is influenced by the presence of mating pheromones, which trigger the mating response in yeast cells.

What are the optimal conditions for Aga2's activity? 05/30/2017

Aga2 exhibits optimum activity at a temperature of 45°C and a pH of 8.0.

Bioinformatic analysis suggests that intrinsic properties of the protein, such as certain dipeptides and the relative volume of aliphatic amino acids, as well as tertiary structural elements like salt bridges, 12/04/2016

How does Aga2's thermostability compare to freezing and thawing? 05/18/2016

Aga2 exhibits good thermostability at temperatures of 45°C and higher, and it retains over 90% of its activity even after undergoing repeated freeze-thaw cycles.

Customer Reviews (5)

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Reviews
05/11/2022

    Protein demonstrated purity levels suitable for sensitive assays

    12/30/2017

       Dependable protein-protein interaction outcomes were enabled by the product’s pure composition.

      11/07/2017

        It enabled clear visualization of cellular structures under the microscope.

        04/13/2017

          The consistent performance of this protein simplified data interpretation.

          03/24/2016

             We attributed the minimal background noise in our assays to the product’s superior quality.

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