Recombinant Saccharomyces Cerevisiae PYC1 Protein (18-470 aa), His-tagged

• Cat.No.: PYC1-878S
• Product Overview: Recombinant Saccharomyces Cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) PYC1 Protein (18-470 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
• Source: E. coli
• Species: Saccharomyces Cerevisiae
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 54.0 kDa
• Protein length: 18-470 aa
• AA Sequence: EKNKILVANRGEIPIRIFRTAHELS MQTVAIYSHEDRLSTHKQKADEAYV IGEVGQYTPVGAYLAIDEIISIAQK HQVDFIHPGYGFLSENSEFADKVVK AGITWIGPPAEVIDSVGDKVSARNL AAKANVPTVPGTPGPIETVEEALDF VNEYGYPVIIKAAFGGGGRGMRVVR EGDDVADAFQRATSEARTAFGNGTC FVERFLDKPKHIEVQLLADNHGNVV HLFERDCSVQRRHQKVVEVAPAKTL PREVRDAILTDAVKLAKECGYRNAG TAEFLVDNQNRHYFIEINPRIQVEH TITEEITGIDIVAAQIQIAAGASLP QLGLFQDKITTRGFAIQCRITTEDP AKNFQPDTGRIEVYRSAGGNGVRLD GGNAYAGTIISPHYDSMLVKCSCSG STYEIVRRKMIRALIEFRIRGVKTN IPFLLTLLTNPVFIEGTYWTTFIDD TPQ
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.
• UniProt ID: P11154

Gene Information

• UniProt ID: P11154