Recombinant SARS-CoV-2 Nucleocapsid Protein, His/AVI-tagged Nucleocapsid-001S

Recombinant SARS-CoV-2 Nucleocapsid Protein, His/AVI-tagged

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Recombinant SARS-CoV-2 Nucleocapsid Protein, His/AVI-tagged

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Cat.No. : Nucleocapsid-001S
Product Overview : Purified Biotinylated recombinant SARS coronavirus 2 Nucleocapsid Protein (N-protein) with N-terminal HIS tag and site-specific Biotinylation on the C-terminal AVI tag was expressed in E.coli.
Description : Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
Source : E. coli
Species : SARS-CoV-2
Tag : His/AVI
Bio-activity : The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Molecular Mass : 49.8 kDa
AA Sequence : MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA
Purity : > 90% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 μg/mL as determined by Bradford protein assay method.
Storage Buffer : 25mM Tris, pH8.0, 250mM NaCl, 10% glycerol.

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