Recombinant SARS-CoV-2 ORF1ab Protein

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Recombinant SARS-CoV-2 ORF1ab Protein

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Cat.No. : ORF1ab-01S
Product Overview : Recombinant SARS-CoV-2 3CL Protease (Ser3264-Gln3569) without tag was expressed in E. coli.
Description : Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies.
Source : E. coli
Species : SARS-CoV-2
Bio-activity : Recombinant SARS-CoV-2 3CL Protease is a cysteine protease that cleaves proteins with sequences including LQ[S/A/G). The cleavage occurs c-terminal to the glutamine residue. Reaction conditions will need to be optimized for each specific application. We recommend an initial SARS-CoV-2 3CL Protease concentration of 20-40 nM when using substrate.
Molecular Mass : 34 kDa
Protein length : 3264-3569
Purity : >95% by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
100 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Storage Buffer : 1.7 mg/mL (50 μM) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM EDTA
Shipping : The product is shipped with dry ice or equivalent.
Inhibition Activity :

GC376 achieves 50% inhibition of 30 nM SARS-CoV-2 3CL Protease at a concentration of 54-71 nM.

SDS-PAGE :

SDS-PAGE of Recombinant SARS-CoV-2 3CL Protease. 1 μg and 2 μg of Recombinant SARS-CoV-2 3CL Protease was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 34 kDa.

Mass Spectrometry :

LC-MS analysis of SARS-CoV-2 3CL Protease. SARS-CoV-2 3CL Protease was analyzed by a Thermo Q Exactive HF instrument then deconvoluted using Thermo BioPharma Finder software. The expected monoisotopic mass is 33774.478 Da.

Gene Name : ORF1ab ORF1a polyprotein;ORF1ab polyprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : ORF1ab
Synonyms : ORF1ab; ORF1a polyprotein;ORF1ab polyprotein; ORF1a polyprotein;ORF1ab polyprotein; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; RNA-dependent RNA polymerase; helicase; 3'-to-5' exonuclease; endoRNAse; 2'-O-ribose methyltransferase; leader protein; nsp2; nsp3; nsp4; 3C-like proteinase; nsp6; nsp7; nsp8; nsp9; nsp10; nsp11; pp1ab; translated by -1 ribosomal frameshift; pp1a
Gene ID : 43740578
Protein Refseq : YP_009725301
UniProt ID : P0DTC1

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