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Recombinant SARS-CoV-2 S Protein, His-tagged
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| Cat.No. : | S-031S |
| Product Overview : | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
| Description : | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests. |
| Source : | HEK293 |
| Species : | SARS-CoV-2 |
| Tag : | His |
| Form : | Liquid |
| Bio-activity : | Measured by its binding ability in a functional ELISA with Human ACE-2. |
| Molecular Mass : | 26.3 kDa |
| AA Sequence : | <DGSM>RVQP TESIVRFPNI TNLCPFGEVF NATRFASVYA WNRKRISNCV ADYSVLYNSA SFSTFKCYGV SPTKLNDLCF TNVYADSFVI RGDEVRQIAP GQTGKIADYN YKLPDDFTGC VIAWNSNNLD SKVGGNYNYL YRLFRKSNLK PFERDISTEI YQAGSTPCNG VEGFNCYFPL QSYGFQPTNG VGYQPYRVVV LSFELLHAPA TVCGPKKSTN LVKNKCVNF< HHHHHH> |
| Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
| Purity : | > 95% by SDS-PAGE |
| Applications : | SDS-PAGE, Bioactivity |
| Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -110 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : | 0.5 mg/mL (determined by Absorbance at 280nm) |
| Storage Buffer : | Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol |
| SDS-PAGE : |
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain |
| Biological Activity : |
SARS-CoV-2 Spike RBD is coated at 5μg/mL (100 μL/well) can bind ACE-2 in a Functional ELISA assay. |
| Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
| Official Symbol : | S |
| Synonyms : | S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein |
| Gene ID : | 43740568 |
| Protein Refseq : | YP_009724390 |
| UniProt ID : | P0DTC2 |
◆ Recombinant Protein
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| S-030S | Recombinant SARS-CoV S Protein, His-tagged | +Inquiry |
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