Recombinant SARS S Protein, Fc-tagged

• Cat.No.: S-673V
• Product Overview: Recombinant receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of rabbit IgG at the C-terminus.

Specification

• Species: SARS
• Source: Insect Cells
• Tag: Fc
• Protein Length: 306-527 a.a.
• Predicted N Terminal: Arg 306
• Form: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 8.0, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike comprises 446 amino acids and has a predicted molecular mass of 50.2 kDa. The apparent molecular mass of the protein is approximately 78.9 and 54.3 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: (36.8+ 56.0) % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: S E2 glycoprotein precursor [ SARS coronavirus ]
• Official Symbol: S
• Synonyms: E2; E2 glycoprotein precursor; spike glycoprotein
• Gene ID: 1489668
• Protein Refseq: NP_828851