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Recombinant SARS S Protein, Fc-tagged

Cat.No.: S-673V
Product Overview: Recombinant receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike (AAX16192.1) (Arg306-Phe527) was expressed with the Fc region of rabbit IgG at the C-terminus.
Source: Baculovirus-Insect cells
Species: SARS
Tag: Fc
Predicted N Terminal: Arg 306
Form: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 8.0, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant receptor binding domain (RBD) of human SARS coronavirus (isolate:WH20) spike comprises 446 amino acids and has a predicted molecular mass of 50.2 kDa. The apparent molecular mass of the protein is approximately 78.9 and 54.3 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: (36.8+ 56.0) % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: S E2 glycoprotein precursor [ SARS coronavirus ]
Official Symbol: S
Synonyms: E2; E2 glycoprotein precursor; spike glycoprotein
Gene ID: 1489668
mRNA Refseq:
Protein Refseq: NP_828851
MIM:
UniProt ID:

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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