Active Recombinant SARS Spike RBD protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: Spike-2433S
• Product Overview: Active Recombinant SARS Spike RBD protein(P59594)(306-527 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: SARS
• Source: HEK293
• Tag: Avi&His
• Protein Length: 306-527 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS Spike RBD, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.