Active Recombinant SARSV Papain-like Protease protein

• Cat.No.: Papain-like Protease-207
• Product Overview: Recombinant SARSV Papain-like Protease(Glu1541 - Lys1841) was expressed in E. coli.

Specification

• Description: The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro. When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species. This protein contains an N-terminal 6-His tag.
• Source: E. coli
• Species: SARSV
• Form: X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
• Bio-activity: Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate. Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well.
• Molecular Mass: Predicted Molecular Mass: 37 kDa
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.