Download Datasheet
See All Papain-like Protease Products| Cat.No.: | Papain-like Protease-207 |
| Product Overview: | Recombinant SARSV Papain-like Protease(Glu1541 - Lys1841) was expressed in E. coli. |
| Description: | The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro. When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species. This protein contains an N-terminal 6-His tag. |
| Source: | E. coli |
| Species: | SARSV |
| Form: | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP |
| Bio-activity: | Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate. Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well. |
| Molecular Mass: | Predicted Molecular Mass: 37 kDa |
| Purity: | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 centigrade as supplied. 3 months, -70 centigrade under sterile conditions after opening. |
Price does not include shipping and packaging costs.Request quote for final price.
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet