Recombinant Shigella Flexneri Serotype X IPAH9.8 Protein (1-545 aa), His-tagged

• Cat.No.: IPAH9.8-1639S
• Product Overview: Recombinant Shigella Flexneri Serotype X (strain 2002017) IPAH9.8 Protein (1-545 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host\'s ubiquitination pathway and modulates the acute inflammatory responses, thus facilitating bacterial colonization within the host cell. Interacts with IKBKG (NO) and TNIP1 (ABIN-1), a ubiquitin-binding adapter protein, which results in TNIP1-dependent \'Lys-27\'-linked polyubiquitination of IKBKG. Consequently, polyubiquitinated IKBKG undergoes proteasome-dependent degradation, which perturbs NF-kappa-B activation during bacterial infection. Uses UBE2D2 (UBCH5B) as an E2 ubiquitin-conjugating enzyme. Mediates polyubiquitination of host U2AF1, leading to its proteasomal degradation.
• Source: Yeast
• Species: Shigella Flexneri Serotype X
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 64.0 kDa
• Protein length: 1-545 aa
• AA Sequence: MLPINNNFSLPQNSFYNTISGTYAD YFSAWDKWEKQALPGEERDEAVSRL KECLINNSDELRLDRLNLSSLPDNL PAQITLLNVSYNQLTNLPELPVTLK KLYSASNKLSELPVLPPALESLQVQ HNELENLPALPDSLLTMNISYNEIV SLPSLPQALKNLRATRNFLTELPAF SEGNNPVVREYFFDRNQISHIPESI LNLRNECSIHISDNPLSSHALQALQ RLTSSPDYHGPRIYFSMSDGQQNTL HRPLADAVTAWFPENKQSDVSQIWH AFEHEEHANTFSAFLDRLSDTVSAR NTSGFREQVAAWLEKLSASAELRQQ SFAVAADATESCEDRVALTWNNLRK TLLVHQASEGLFDNDTGALLSLGRE MFRLEILEDIARDKVRTLHFVDEIE VYLAFQTMLAEKLQLSTAVKEMRFY GVSGVTANDLRTAEAMVRSREENEF TDWFSLWGPWHAVLKRTEADRWAQA EEQKYEMLENEYPQRVADRLKASGL SGDADAEREAGAQVMRETEQQIYRQ LTDEVLALRLSENGSQLHHS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: ipaH9.8 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 [ Shigella flexneri 5a str. M90T ]
• Official Symbol: IPAH9.8
• Synonyms: ipaH9.8; Invasion plasmid antigen ipaH9.8
• Gene ID: 40047555
• Protein Refseq: WP_000936806
• UniProt ID: D2AJU0