Recombinant Streptococcus pyogenes Cas9 Protein, His-tagged

• Cat.No.: Cas9-02S
• Product Overview: Recombinant Cas9-NLS comprises the entire Cas9 protein sequence (1368 amino acids) fused to a proprietary nuclear localization sequence (NLS) and a 6 X His tag at the C-terminus was expressed in E. coli
  Features: Low endotoxin Level, High Biological activity, Consistent from lot to lot.

Specification

• Species: Streptococcus pyogenes
• Source: E.coli
• Tag: His
• Molecular Mass: 162 kDa
• Purity: Greater than 97 % as determined by SDS-PAGE.
• Applications: Genome editing
• Storage: At -20 centigrade.
• Storage Buffer: 10 X Cas9 Reaction Buffer: 200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5
  Storage Buffer: 50 mM Tris-HCl, 50 M KCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 7.5

Gene Information

• Official Symbol: Cas9
• Synonyms: Cas9; CRISPR associated protein 9
• UniProt ID: Q99ZW2