Recombinant Streptomyces avidinii Protein, His tagged

• Cat.No.: Streptavidin-041S
• Product Overview: Recombinant Streptomyces avidinii Streptavidin protein (P22629) (Gly 38-Ser 163) with a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Streptomyces avidinii
• Source: E.coli
• Tag: His
• Protein Length: 38-163aa
• Description: Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.
• Tag: C-His
• Molecular Mass: 14.9 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Official Symbol: SA
• Synonyms: Streptavidin
• UniProt ID: P22629