Recombinant Stromal Interaction Molecule 1

• Cat.No.: STIM1-4619H
• Product Overview: Recombinant STIM1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids and having a molecular mass of 38 kDa. STIM1 is fused to Calmodulin and purified by conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: STIM1 is a type-1 transmembrane protein that is necessary for store-operated Ca(2+) entry, a process of extracellular Ca(2+) influx in response to the depletion of Ca(2+) stores in the endoplasmic reticulum (ER). STIM1 localizes predominantly to the ER; upon Ca(2+) release from the ER, STIM1 translocates to the ER-plasma membrane junctions and activates Ca(2+) channels. STIM1 is an adhesion molecule involved in early hematopoiesis by mediating attachment to stromal cells. STIM1 controls the survival and/or proliferation of b-cell precursors.
• Form: The STIM1 protein solution (1mg/ml) contains 20mM Tris pH-7.5.
• Purity: Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
• Pathways: SNARE interactions in vesicular transport; Membrane Trafficking

Gene Information

• Gene Name: STIM1 stromal interaction molecule 1 [ Homo sapiens ]
• Official Symbol: STIM1
• Synonyms: STIM1; stromal interaction molecule 1; STIM1 stromal interaction molecule 1; GOK; D11S4896E
• Gene ID: 6786
• mRNA Refseq: NM_003156
• Protein Refseq: NP_003147
• MIM: 605921
• UniProt ID: Q13586
• Chromosome Location: 11p15.5
• Function: calcium ion binding; protein binding