Recombinant Thermus aquaticus polA protein

• Cat.No.: polA-4486T
• Product Overview: Recombinant Thermus aquaticus polA protein(P19821)(293-832aa(I614,L616I)) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Thermus aquaticus
• Tag: N/A
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 61.0 kDa
• Protein length: 293-832aa(I614,L616I)
• AA Sequence: ALEEAPWPPPEGAFVGFVLSRKEPM WADLLALAAARGGRVHRAPEPYKAL RDLKEARGLLAKDLSVLALREGLGL PPGDDPMLLAYLLDPSNTTPEGVAR RYGGEWTEEAGERAALSERLFANLW GRLEGEERLLWLYREVERPLSAVLA HMEATGVRLDVAYLRALSLEVAEEI ARLEAEVFRLAGHPFNLNSRDQLER VLFDELGLPAIGKTEKTGKRSTSAA VLEALREAHPIVEKILQYRELTKLK STYIDPLPDLIHPRTGRLHTRFNQT ATATGRLSSSDPNLQNIPVRTPLGQ RIRRAFIAEEGWLLVALDYSQNEIR VLAHLSGDENLIRVFQEGRDIHTET ASWMFGVPREAVDPLMRRAAKTINF GVLYGMSAHRLSQELAIPYEEAQAF IERYFQSFPKVRAWIEKTLEEGRRR GYVETLFGRRRYVPDLEARVKSVRE AAERMAFNMPVQGTAADLMKLAMVK LFPRLEEMGARMLLQVHDELVLEAP KERAEAVARLAKEVMEGVYPLAVPL EVEVGIGEDWLSAKE
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.