|Description :||Recombinant Treponema pallidum 17Kd protein produced as a P-fusion protein in the E.coli Expression System. Fusion protein protein mol.wt: approx.19kD. This protein binds to murine anti-TP17 kD protein monoclonal antibodies and Treponema pallidum converted human serum polyclonal antibodies in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.|
|Species :||Treponema Pallidum|
|Form :||Frozen Liquid|
|Molecular Mass :||19 kD|
|Purity :||> 95 % by SDS-PAGE|
|Applications :||In-Vitro Diagnostics;
|Notes :||Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.
|Stability :||At least 2 years at -75 centigrade.|
|Storage :||Store at -75 centigrade.|
|Dilutions :||10 mM Tris, pH 8.0, SDS (<0.01%)|