Active Recombinant Viral B18R Protein

• Cat.No.: B18R-174
• Product Overview: Recombinant viral B18R (His20-Glu351) protein with Fc region of human Human IgG1 (Pro100-Lys300) at N-terminus was expressed in Human embryonic kidney cell, HEK293.

Specification

• Description: Counteracts the antiviral effects of host IFN-alpha/beta. Acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.
• Source: HEK293
• Species: Viral
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. The ED50 for this effect, as measured by inhibition of recombinant Human IFN‑ alpha 2, is 0.3-1.8 ng/mL.
• Molecular Mass: 65 kDa
• N-terminal Sequence Analysis: Met-Asp-Pro100
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Preservative: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: B19R temporal expression: early [ Vaccinia virus ]
• Official Symbol: B19R
• Synonyms: IFN-alpha/beta-receptor-like secreted glycoprotein; similar to VACCP-B19R; host defense modulator [VACWR-B18R]; B18R; B19R
• Gene ID: 3707577
• UniProt ID: P25213